Novel Biosynthesis of Graphene-Supported Zero-Valent Iron Nanohybrid for Efficient Decolorization of Acid and Basic Dyes

Herein, respiratory nitrate reductases (NAR) were utilized in the biosynthesis of zero-valent iron (ZVI) graphene nanocomposite as a simultaneous reducing and capping agent, for first time, to efficiently adsorb methylene blue (MB) direct red-81 (DR-81). Under anaerobic conditions, greenly synthesized was incubated with precursor presence crude-NAR enzyme 48 h obtain ZVI composite followed by characterizing this using physiochemical analyses. Scanning transmission electron microscopy, energy dispersive X-ray spectroscopy diffraction techniques assured chemical composition interaction between graphene. The influences operating conditions such contact pH adsorbent dose on adsorption efficacy explored case graphene, ZVI. displayed highest removal efficiency MB DR-81 compared ZVI-NPs. percentages nanocomposites 88.3 ± 2.66% 87.6 2.1%, respectively, at 7, 20 mg/50 mL, initial or concentration 10 mg/L shaking speed 150 rpm. A pseudo first-order model could describe kinetics, mechanism discussed. promising results current study support potential recruitment eliminating various pollutants from industrial effluents larger scale. Further, prepared nanohybrid can be used other applications photocatalysis, Fenton persulfate activation processes.